| W | World | 43.03 | Wayde van Niekirk | South Africia | 8/14/2016 | Rio de Janerio, BRA |
| A | American | 43.18 | Michael Johnson | Nike | 8/26/1999 | Seville, ESP |
| C | College Outdoor | 44.00 | Quincy Watts | So. California | 6/6/1992 | Austin, TX |
| Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|
| 1 | TUCKER, TRAVIS | SR | SENATOBIA | 49.86 |
| 2 | WEBB, JAQUAN | JR | OXFORD (MS) | 50.00 |
| 3 | WRIGHT, TAVARIUS | SR | OLIVE BRANCH | 50.10 |
| 4 | HONORABLE, JAKIEL | JR | OLIVE BRANCH | 52.14 |
| 5 | YOUNG, NIAGERIA | SO | SENATOBIA | 52.50 |
| 6 | CALHOON, KEATON | JR | OXFORD (MS) | 52.96 |
| 7 | POLK, RONALD | JR | PICAYUNE | 53.74 |
| 8 | HOUSE, DEONDRE | SO | SENATOBIA | 53.98 |
| 9 | HARRIS, TRACY | JR | W. TALLAHATCHIE | 55.31 |
| 10 | MAGEE, CHRIS | SR | PICAYUNE | 55.34 |
| 11 | PEGUES, WESLEY | FR | OXFORD (MS) | 55.86 |
| 12 | MAVBELL, RYAN | SR | SIMMONS | 55.88 |
| 13 | WILLIAMS, MARKEION | JR | INDEPENDENCE | 56.06 |
| 14 | PARKS, KAMDEN | JR | AMORY | 56.78 |
| 15 | MARSHALL, ASHER | JR | LAKE CORMORANT | 56.80 |
| 16 | WATSON, MEKAL | JR | INDEPENDENCE | 58.00 |
| 17 | MCINTOSH, MICHAEL | FR | CORINTH | 58.20 |
| 18 | CHANDLER, CARTER | JR | CORINTH | 58.50 |
| 19 | BYRD, CAREY | SR | PICAYUNE | 59.63 |
| 20 | SEALS, LEVI | JR | CORINTH | 1:00.25 |
| 21 | BERKLEY, KOBE | JR | DESOTO CENTRAL | NT |
| 22 | MALONE, TAVARIS | SR | OLIVE BRANCH | NT |
| 23 | ROBINSON, EDDIE | SO | DESOTO CENTRAL | NT |
| 24 | POWELL, ELIJAH | SR | W. TALLAHATCHIE | NT |
| 25 | LITTLE, LAMARCUS | 8 | SIMMONS | NT |
| 26 | WOODS, JORDAN | SR | DESOTO CENTRAL | NT |
| 27 | BETHANY, CHARLES | 8 | SIMMONS | NT |
| 28 | NELSON, KEVIN | FR | LAKE CORMORANT | NT |