| W | World | 3:15.17 | Ledovskaya, Nazarova, Pinigina, Bryzgina | URS | 10/1/1988 | Seoul, KOR |
| A | American | 3:15.51 | Howard, Dixon, Brisco, Joyner | USA national team | 10/1/1988 | Seoul, KOR |
| C | College Outdoor | 3:23.75 | Jones, McIntosh, Chapple, Richards | Texas | 4/3/2004 | Austin, TX |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | DESOTO CENTRAL | A | Kennedy, Tylah | Seals, Kenia | Wofford, Dioncia | Bulloch, Kiara | NT |
| 2 | INDEPENDENCE | A | Lee, Khiara | Reynolds, Arkiyah | Roberson, Cameron | Watts, Deavion | NT |
| 3 | CORINTH | A | Lopez, Lesley | Spence, Adaysha | Taylor, Sentaria | Burns, Callie | NT |
| 4 | SENATOBIA | B | Allen, Ebony | Deberry, Jasmine | Miller, Alandria | Rhone, Casey | DQ |
| 5 | OXFORD (MS) | A | Dunn, Jakiara | Elliott, Swayze | Epps, Ashley | Ramsey, Kadijah | 4:11.72 |
| 6 | SENATOBIA | A | Burdette, Shakiya | Richardson, Athena | Cathey, Destini | Blanks, Alexis | 4:44.00 |
| 7 | SIMMONS | A | Davis, Tishuna | Greer, Anisha | Maybell, Nicole | White, Rikeshia | NT |
| 8 | W. TALLAHATCHIE | A | Anderson, Shakayla | Jenkins, Aaliyah | Pimpton, Zyerria | Spraggin, Dyiesha | DNF |
| 9 | OLIVE BRANCH | A | Moore, Haley | Puryear, Madison | Thomas, Coriana | Piacentini, Yamileth | DNS |