| W | World | 43.03 | Wayde van Niekirk | RSA | 8/14/2016 | Rio de Janerio, BRA |
| A | American Outdoor | 43.18 | Michael Johnson | Nike | 8/26/1999 | Seville, ESP |
| C | College Outdoor | 43.61 | Michael Norman | So. California | 6/8/2018 | Eugene, OR |
| S | School | 47.02 | DeAndra Lipscomb | 2014 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 350 | ABIAKAM, JOSEPH | SR | NORFOLK STATE |
NT | 52.10 |
| 3 | 91 | KREHNBRINK, COLE | FR | APP. STATE |
49.30 | Bill Webb Multi/Bob Davidson Invite |
| 4 | 332 | HAYES, SAVIEN | JR | NORFOLK STATE |
48.84 | Darius Dixon Memorial Invite 2020 |
| 5 | 159 | KOVAR, BRENDAN | FR | COASTAL CAROLINA |
48.98 | South Carolina Invitational |
| 6 | 60 | DIXON JR., THOMAS | FR | APP. STATE |
50.66 | Bill Webb Multi/Bob Davidson Invite |
| 7 | 151 | HARRIS, DESHON | FR | COASTAL CAROLINA |
52.81 | Alan Connie Shamrock Invitational |
| 8 | 356 | FRANKLIN, ANTHONY | JR | NORFOLK STATE |
NT | 51.00 |