| W | World Outdoor 2021 | 3:15.17 | Ledovskaya, Nazarova, Pinigina, Bryzgina | URS | 10/1/1988 | Seoul, KOR |
| A | American Outdoor 2021 | 3:15.51 | Howard, Dixon, Brisco, Joyner | USA national team | 10/1/1988 | Seoul, KOR |
| C | College Outdoor 2021 | 3:23.13 | Dunmore, Stevens, Guster, Rogers | Oregon | 6/10/2017 | Eugene, OR |
| U | C-USA championship | 3:34.76 | Lee, Petty, Singletary, Williams | Charlotte | 5/12/2019 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | RICE |
A | Simms | Bangala | Hunter-Galvan | Kadmon | 3:54.62 |
| 4 | SO. MISS. |
A | Varnell | Griffin | Harris | Benson | 3:50.45 |
| 5 | F.I.U. |
A | Clark | Cuenca | Lubeskie | Forde-Richards | 3:49.36 |
| 6 | CHARLOTTE |
A | Fairman | Core | Petty | Singletary | 3:49.51 |
| 7 | LA TECH |
A | Masters | Edwards | Murray | Watson | 3:54.14 |
| 8 | FL. ATLANTIC |
A | Simpson | Francois | King | Lambert | 4:06.82 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | MARSHALL |
A | Murphy | Alleyne | Warner | White | 3:48.63 |
| 4 | NORTH TX |
A | BURNHAM | CHOICE | Collins | SCOTT | 3:47.21 |
| 5 | A | Akanwogba | Abrocquah | Nyenke | Obi | 3:41.77 | |
| 6 | UAB |
A | Green | Tolliver | Harris | Petkov | 3:44.07 |
| 7 | UTSA |
A | Chinedu | Lomax | Armstrong | Yukich | 3:48.43 |
| 8 | UTEP |
A | Brown | Brume | Roberts | Chepkosgei | 3:48.93 |