| W | World Outdoor | 36.84 | Carter, Frater, Blake, Bolt | Jamaica | 8/11/2012 | London, ENG |
| A | American Outdoor | 37.38 | Rodgers, Gatlin, Gay, Bailey | USA national team | 5/2/2015 | Nassau, BAH |
| A | American Outdoor | 37.38 | Demps, Patton, Kimmons, Gatlin | USA national team | 8/10/2012 | London, ENG |
| C | College Outdoor | 37.97 | Ekevwo, Sani-Brown, Holloway, Clark | Florida | 6/7/2019 | Austin, TX |
| N | NEC championship | 40.24 | N Mahlung, B Richards, B Rodney, M Williamson | LIU Brooklyn | 5/5/2013 | W Long Branch, NJ |
| I | IC4A | 42.50 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | SACRED HEART |
A | Koproski | Young | Duncanson | Pelletier | 43.75 | 43.75 2021 Mount Invitational |
| 5 | ST. FRANCIS (N.Y.) |
A | Hernandez | Theosmy | Myers | Strachn | 43.43 | 43.43 New Jersey Relays |
| 6 | MERRIMACK |
A | Jasseh | Rahaman | Hughes | Loreus | 43.51 | 43.51 Merrimack College Invitational |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | MOUNT ST. MARY'S |
A | Tarawali | Kungu | Okoye | Aguoru | 42.11 | 42.11 2021 Mount Invitational |
| 3 | ST. FRANCIS UNIV |
A | Baker | Ndeh | Williams | McFadden | 41.95 | 41.95 Bill Webb Multi/Bob Davidson Invite |
| 4 | FAIRLEIGH DICKINSON |
A | Oglesby | Elysee | Mane | O'Garro | 41.70 | 41.70 2021 Monmouth Spring Invitational |
| 5 | LIU |
A | Harding | Sey | Fontes | McKenzie | 41.14 | 41.14 SFU Red Flash Invitational |
| 6 | CENTRAL CONNECTICUT |
A | Dixon | Diaby | Whalen | Fye-Moore | 41.39 | 41.39 Bryant University vs CCSU%2C URI |
| 7 | BRYANT |
A | Ferraiola | Goodman | Cardarelli | Smith | 41.95 | 41.95 Bryant U vs American U%2C Brown U |
| 8 | WAGNER |
A | Lane | Cadet | Cadet | Babcanec | 42.02 | 42.02 2021 Mount 33-48 Invitational |