| W | World Outdoor 2021 | 36.84 | Carter, Frater, Blake, Bolt | Jamaica | 8/11/2012 | London, ENG |
| A | American Outdoor 2021 | 37.38 | Demps, Patton, Kimmons, Gatlin | USA national team | 8/10/2012 | London, ENG |
| A | American Outdoor 2021 | 37.38 | Rodgers, Gatlin, Gay, Bailey | USA national team | 5/2/2015 | Nassau, BAH |
| C | College Outdoor 2021 | 37.97 | Ekevwo, Sani-Brown, Holloway, Clark | Florida | 6/7/2019 | Austin, TX |
| OV | OVC championship | 39.79 | Boey, Edwards, Robinson, Mohammed | E. Illinois | 2015 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | SE MISSOURI |
A | Schwab | Wood | Lay | Mathuthu | NT | |
| 3 | EASTERN KENTUCKY |
A | Carter | Favors | Sawyers | Jackson | DQ | 2021 Kentucky Invtational |
| 4 | SIU EDWARDSVILLE |
A | Brown | Bretz | Knapp | Nuzzo | 42.08 | 2021 Joey Haines Invite |
| 5 | EASTERN ILLINOIS |
A | Johnson | Powell | Mhonde | Johnson | 40.35 | Pacesetter Sports Invitational |
| 6 | TENNESSEE ST. |
A | Petty | Redmon | Warren | Brumfield | 41.44 | Georgia Tech Invitational |
| 7 | BELMONT |
A | Stover | Sheppard | Hodge | Arnold | 42.11 | Vanderbilt Black & Gold Invitational |
| 8 | TENNESSEE-MARTIN |
A | Martin | Smith | Hayes | Holland | 42.44 | Tennessee Challenge |