| W | World Outdoor 2021 | 40.82 | Bartoletta, Felix, Knight, Jeter | USA | 8/11/2012 | London, ENG |
| A | American Outdoor 2021 | 40.82 | Bartoletta, Felix, Knight, Jeter | USA national team | 8/10/2012 | London, ENG |
| C | College Outdoor 2021 | 42.05 | Brisco, Johnson, Misher, Hobbs | L.S.U. | 5/13/2018 | Knoxville, TN |
| OV | OVC championship | 45.00 | Tate, Robinson, Jackson, Wickham | Tennessee Tech | 5/12/2018 | Cookeville, TN |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | SIU EDWARDSVILLE |
A | Hajderovic | Sullivan | Bivins | Carter | 49.90 | Big Blue Classic |
| 5 | MOREHEAD STATE |
A | Andrews | Harvey | Kelley | Lee | 49.18 | 2021 Kentucky Invtational |
| 6 | TENNESSEE TECH |
A | Tate | Anderson | Allen | Vance | 49.39 | 49.39 Tennessee Challenge |
| 7 | JACKSONVILLE ST. |
A | Raggs | Rollins | Coprich | Lee | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | SE MISSOURI |
A | James | Williams | Cogio | Miles | 48.03 | Tennessee Relays |
| 4 | EASTERN ILLINOIS |
A | Coleman | Frank | Johnson | Kollore | 46.12 | Drake Relays |
| 5 | MURRAY STATE |
A | Denning | James | Herrero-Yanez | Seals | 45.14 | Vanderbilt Black & Gold Invitational |
| 6 | EASTERN KENTUCKY |
A | Smith | Perry | Roesser | Scott | 45.69 | North Florida Invite |
| 7 | TENNESSEE ST. |
A | Townsend | Coleman | Johnson | Henry | 47.85 | Georgia Tech Invitational |
| 8 | BELMONT |
A | Barlow | Balcerak | Pruitt | Quigley | 48.62 | Vanderbilt Black & Gold Invitational |