| W | World Outdoor 2021 | 3:15.17 | Ledovskaya, Nazarova, Pinigina, Bryzgina | URS | 10/1/1988 | Seoul, KOR |
| A | American Outdoor 2021 | 3:15.51 | Howard, Dixon, Brisco, Joyner | USA national team | 10/1/1988 | Seoul, KOR |
| C | College Outdoor 2021 | 3:23.13 | Dunmore, Stevens, Guster, Rogers | Oregon | 6/10/2017 | Eugene, OR |
| OV | OVC championship | 3:37.74 | Robinson, Tate, Jackson, Roberts | Tennessee Tech | 5/12/2018 | Cookeville, TN |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | EASTERN KENTUCKY |
A | Scott | Garcia Palencia | Roesser | Barr | 4:16.17 | 4:16.17 2021 Kentucky Invtational |
| 5 | TENNESSEE TECH |
A | Tate | Akili | Vance | Stephens | 4:09.95 | 4:09.95 WKU Hilltopper Relays |
| 6 | TENNESSEE ST. |
A | Townsend | Henry | Johnson | Coleman | 4:15.13 | UCF Black and Gold Invite |
| 7 | TENNESSEE-MARTIN |
A | Taylor | Medley | Mccullough | Plotner | 4:28.25 | 2021 Joey Haines Invite |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | SIU EDWARDSVILLE |
A | Carter | Horton | Krone | Hajderovic | 4:04.32 | Illinois State Invitational |
| 3 | SE MISSOURI |
A | Miles | Long | James | Morrill | 3:53.41 | Tennessee Relays |
| 4 | EASTERN ILLINOIS |
A | Stinson | Adams | Kollore | Johnson | 3:51.05 | Drake Relays |
| 5 | MURRAY STATE |
A | McSwain | Herrero-Yanez | Thornton | Bell | 3:44.76 | 2021 Kentucky Invtational |
| 6 | AUSTIN PEAY |
A | Johnson | Smith | Phillips | Thomasson | 3:49.99 | 2021 Kentucky Invtational |
| 7 | BELMONT |
A | Pruitt | Richmond | Quigley | Barlow | 3:53.03 | 2021 Joey Haines Invite |
| 8 | JACKSONVILLE ST. |
A | Coats | Register | Porch | Coprich | 4:00.90 | GATA Classic |
| 9 | MOREHEAD STATE |
A | Kelley | Andrews | Camacho | Jones | 4:06.95 | EKU Rick Erdmann Twilight |