| W | World Outdoor | 43.03 | Wayde van Niekerk | RSA | 8/14/2016 | Rio de Janeiro, BRA |
| A | American Outdoor | 43.18 | Michael Johnson | Nike | 8/26/1999 | Seville, ESP |
| C | College Outdoor | 43.61 | Michael Norman | So. California | 6/8/2018 | Eugene, OR |
| VT | Vanderbilt Track | 45.51 | Kelly Willie | LSU | 5/15/2005 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ARNOLD, ISHMAEL | SR | BELMONT |
48.83 | Joe Walker Invitational |
| 3 | FULLER, QUA'JON | FR | BELMONT |
47.88 | Joe Walker Invitational |
| 4 | UHL, JACOB | JR | BUTLER |
47.75 | Raleigh Relays |
| 5 | JOHNSON, WILLIE | FR | SO ILLINOIS |
47.60 | Ole Miss Classic |
| 6 | BRYANT, NICK | FR | SE MO. |
47.88 | Tennessee Relays |
| 7 | SCHWAB, COLLIN | JR | SE MO. |
48.25 | OVC Championship |
| 8 | SHEPPARD, ELIJAH | JR | BELMONT |
49.27 | Georgia Tech Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | JOHNSON, JEREMY | SR | 52.57 | Oliver Nikoloff Invitational - 2021 | |
| 3 | HAYES, MARQUERIOUS | SR | TN-MARTIN |
51.90 | 2021 Joey Haines Invite |
| 4 | SMITH, COREY | FR | TN-MARTIN |
50.01 | Margaret Simmons Invite |
| 5 | ENGLISH, LUKE | SR | 49.49 | Blizzard Buster | |
| 6 | NATIONS, GUS | SO | 51.18 | University of Akron Campbell Invitational | |
| 7 | MULLEN, ROBERT | AUSTIN PEAY |
52.42 | WKU Hilltopper Relays | |
| 8 | GRIFFIN, HENRY | FR | TN-MARTIN |
NT | 52 |