| W | World Outdoor | 45.94 | Karsten Warholm | NOR | 8/3/2021 | Tokyo, JPN |
| A | American Outdoor | 46.17 | Rai Benjamin | Nike | 8/3/2021 | Tokyo, JPN |
| C | College Outdoor 1 | 47.56 | Rai Benjamin | So. California | 6/8/2018 | Eugene, OR |
| S | SWU/ School | 53.84 | Jhante McMillan | 5/22/2014 | Gulf Shores, AL |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | BROOKS, ZANE | FR | ERSKINE |
1:01.55 |
| 2 | KEY, JAXON | FR | ERSKINE |
1:01.02 |
| 3 | GRAY, MISHA | SO | 59.03 | |
| 4 | TUCKER, LANDON | FR | NEWBERRY |
57.07 |
| 5 | FRANCIS, KEVIN | SR | BENEDICT |
53.51 |
| 6 | BARTLETT, RASHANE | JR | LIMESTONE |
53.87 |
| 7 | MOSS, JABARI | SO | ALLEN |
57.24 |
| 8 | ORTON, TEDDY | FR | MARYVILLE (TENN.) |
1:00.50 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | SMART, ZION | FR | ALLEN |
NT |
| 2 | BROWN, SHAMAR | SR | NEWBERRY |
NT |
| 3 | DUNN, JAYDEN | FR | ALLEN |
NT |
| 4 | POWELL, ELIJAH | FR | SOUTHERN WESLEYAN |
1:03.24 |
| 5 | NICOL, DAVIS | SR | MONTREAT |
1:01.59 |
| 6 | SIMPKINS, JADEN | FR | 1:02.81 | |
| 7 | PURVIS, NATHAN | FR | 1:07.98 | |
| 8 | MADDOX, ALIJAH | FR | ALLEN |
NT |