| W | World Outdoor | 49.59 | Jamila Kratochvilova | TCH | 3/7/1982 | Milan, Spain |
| A | American Outdoor | 48.70 | Sandi Richards | Nike | 9/17/2006 | Athens, GRE |
| C | College Outdoor 1 | 49.71 | Courtney Okolo | Texas | 4/23/2016 | Baton Rouge, LA |
| S | SWU/ School | 54.43 | Jami Wright | 2021 | Greensboro, NC |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 2 | HYDE, ANGELLYN | SO | 1:01.06 | |
| 3 | JONES, MEASHA | SO | ALLEN |
1:00.85 |
| 4 | NOAD, TORRIANN | SO | BENEDICT |
58.20 |
| 5 | DEADWYLER, EMMA | JR | ANDERSON (S.C.) |
58.00 |
| 6 | MOSES, KANESHA | JR | BENEDICT |
58.00 |
| 7 | MACURDA, ALLY | FR | ANDERSON (S.C.) |
59.50 |
| 8 | THOMPSON, KATHERINE | SR | ERSKINE |
1:01.00 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | LAY, TRAVON | JR | SOUTHERN WESLEYAN |
1:03.31 |
| 2 | WALKER, KETURAH | FR | ALLEN |
1:03.00 |
| 3 | MANTERNACH, KAYLENE | FR | MONTREAT |
1:02.76 |
| 4 | DAVIS, ALYSE | FR | SOUTHERN WESLEYAN |
1:02.49 |
| 5 | DIANISO, MIA | JR | NEWBERRY |
1:01.11 |
| 6 | LOGUE, ZOE | SR | EMMANUEL (GA.) |
1:01.82 |
| 7 | MARKS, ALYSSA | SR | 1:02.65 | |
| 8 | TATE, LAURYN | JR | SOUTHERN WESLEYAN |
1:02.85 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | WILLIAMS, ASHLEY | JR | EMMANUEL (GA.) |
NT |
| 2 | KING, KENYA | JR | NEWBERRY |
NT |
| 3 | CRAWFORD, ANDREA | FR | NT | |
| 4 | ARMINTROUT, SHELBY | SR | MARYVILLE (TENN.) |
NT |
| 5 | WALTON, EMILY | SO | 1:03.67 | |
| 6 | BARNETT, CAVEL | SO | BENEDICT |
1:05.00 |
| 7 | BAXTER, ALONIE | SR | JOHNSON & WALES (N.C.) | NT |
| 8 | GUSKO, EMMA | FR | NT |