| W | World Outdoor 2025 | 43.03 | Wayne van Niekerk | RSA | 8/14/2016 | Rio de Jameiro, BRA |
| A | American Outdoor 2025 | 43.18 | Michael Johnson | Nike | 8/26/1999 | Seville, ESP |
| C | College Outdoor 2025 | 43.61 | Michael Norman | So. California | 6/8/2018 | Eugene, OR |
| H | Horizon League | 46.69 | Tyler Bucholz | Milwaukee | 2010 |
| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 1 | 677 | DELBAUGH, WILLIAM | SR | YOUNGSTOWN ST. |
51.04 |
| 2 | 473 | NORTHINGTON, CAINEN | FR | PURDUE FORT WAYNE |
50.00 |
| 3 | 470 | MORRIS, NOAH | FR | PURDUE FORT WAYNE |
49.78 |
| 4 | 91 | MCKENZIE, JADEN | JR | DETROIT MERCY |
48.36 |
| 5 | 469 | MORRIS, JONAS | SO | PURDUE FORT WAYNE |
49.28 |
| 6 | 208 | BUFORD, ANTHONY | FR | MILWAUKEE |
49.98 |
| 7 | 302 | KOROLY, BRANTON | FR | NORTHERN KENTUCKY |
50.11 |
| 8 | 299 | HARRISON, ELIJAH | FR | NORTHERN KENTUCKY |
50.87 |
| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 2 | 677 | DELBAUGH, WILLIAM | SR | YOUNGSTOWN ST. |
49.34 |
| 3 | 299 | HARRISON, ELIJAH | FR | NORTHERN KENTUCKY |
50.41 |
| 4 | 208 | BUFORD, ANTHONY | FR | MILWAUKEE |
49.49 |
| 5 | 302 | KOROLY, BRANTON | FR | NORTHERN KENTUCKY |
50.60 |
| 6 | 469 | MORRIS, JONAS | SO | PURDUE FORT WAYNE |
47.70 |
| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 2 | 91 | MCKENZIE, JADEN | JR | DETROIT MERCY |
48.32 |
| 3 | 83 | FERGUSON, MICHAEL | SO | DETROIT MERCY |
51.11 |
| 4 | 470 | MORRIS, NOAH | FR | PURDUE FORT WAYNE |
48.67 |
| 5 | 240 | SCHINDLER, KIERAN | FR | MILWAUKEE |
50.19 |
| 6 | 473 | NORTHINGTON, CAINEN | FR | PURDUE FORT WAYNE |
49.95 |