| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | SCOTT'S BRANCH | A | Traonha | Pearson | Ford | Taylor | DNS |
| 2 | CARVERS BAY | B | Beckman | Britton | Hemingway | Vereen | NT |
| 3 | MANNING | A | Griffin | Henry | Wilder | Bennett | 54.38 |
| 4 | ANDREWS HIGH SCHOOL | A | George | Gibson | Wallace | Hurell | 50.44 |
| 5 | LAKE CITY | A | Ford | Ford | Gregg | Muldrow | 51.65 |
| 6 | CARVERS BAY | A | Smith | Hutchinson | Wilson | Holmes | NT |
| 7 | HEMINGWAY | A | Hayward | Bell | Singletary | Cooper | DNS |
| 8 | MULLINS | A | Bell | Ford | Boyd | Gillard | DNS |