| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | MULLINS | A | Reaves | Grant | Mills | Bailey | DNS |
| 2 | ANDREWS HIGH SCHOOL | A | Belin | Flagler | Lee | Shaw | NT |
| 3 | CARVERS BAY | A | Bell | Beckman | Rush | Keith | 47.05 |
| 4 | MANNING | A | Green | Canty | Hayward | Canty | 45.09 |
| 5 | LAKE CITY | A | Scott | Jones | Singletary | Matthews | 45.62 |
| 6 | CARVERS BAY | B | Diggs-Perkins | Young | Davis | Britton | DNS |
| 7 | ANDREWS HIGH SCHOOL | B | McCray | Scott | Smalls | Rolle | DNS |
| 8 | HEMINGWAY | A | Hannah | Timmons | Nesmith | Cooper | DNS |