| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | JOHNSONVILLE | A | Wright | Woodbury | Livingston | Geter | NT |
| 3 | ANDREWS HIGH SCHOOL | A | McCray | Murphy | White | McCullough | 4:27.55 |
| 4 | CARVERS BAY | A | Linen | Britton | Yokwe | Diggs-Perkins | 3:48.42 |
| 5 | LAKE CITY | A | Smith | Thomas | Brayboy | Boone | 4:04.49 |
| 6 | KINGSTREE MIDDLE MAGNET SCHOOL O | A | Epps | Johnson | Cooper | Pressley | 4:47.95 |
| 7 | CONWAY | A | NT |